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KMID : 1143520180060020053
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Journal of Korean Society of Oral Health Science
2018 Volume.6 No. 2 p.53 ~ p.58
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Regulation effects of orofacial pain by green coffee bean extracts in rats
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Lee Min-Kyung
Kim Hee-Jin
Park Min-Kyung
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Abstract
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Green coffee bean (GCB) is a raw coffee bean that has not yet been roasted. Green coffee bean is the unroasted or immature robust coffee bean. It is mostly composed of Chlorogenic Acids (CGA), caffeine, trigonelline, and the diterpenes cafestol and kahweol. Chlorogenic Acids (CGA) have the anti-cancer effect of restraining free oxygen radicals. Even though experiments using Green Coffee Bean Exract (GCBE) have been done in various fields, experiments with regard to orofacial pain have been few. The aim of this study is to investigate whether administration of GCBE may be beneficial in pain modulation in inflammatory orofacial pain. We investigated by using GCBE in the development and modulation of orofacial pain in rats. Male, 7- to 8-week-old, Sprague-Dawley rats weighing 240-280g were used in this study. Experiments were carried out using a subcutaneous (SC) pain model caused by the injection of 5% formalin into the right vibrissa pad (SC, 50 μl). The amount of scratching or rubbing to the injection site was recorded for 9 consecutive 5-minute intervals following injection of formalin. The experimental subjects were separated into 5 groups (n=4); Naive, formalin(5%), formalin after distilled water (vehicle), formalin after GCBE (p.o.,concentrations of 30,60mg/mL). The number of behavioral reactions of scratching the facial region proximal to the injection site was recorded for 9 consecutive 5-minute intervals following injection of the formalin. As a result, we found that oral administration of GCBE (p.o., concentrations of 30, 60 mg/mL) has an effect of relieving the formalin-induced orofacial pain. In particular, high concentrations (60 mg/mL) of GCBE substantially decrease the nociceptive behavior. These results mean that GCBE may affect the occurrence of orofacial pain and modulation. Therefore, Green coffee bean extract may be a potential therapeutic treatment for orofacial pain.
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KEYWORD
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Green coffee bean, Orofacial pain, Formalin
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